Description

Fetal Bovine Serum (FBS) Activated Charcoal Absorbed is produced by filtering FBS through an activated carbon-absorbent filter, resulting in reduced concentrations of steroid hormones, such as estradiol, progesterone, cortisol, testosterone, T3, and T4, without unspecific loss of other serum components. FBS Charcoal Stripped is useful for studying processes influenced by steroid hormones (estrogen stimulation, obesity process) and other research topics that benefit from reduced hormone levels, such as certain types of viral infections.

Fetal bovine serum (FBS) or fetal calf serum (FCS) is widely used in eukaryotic cell culture, typically as a supplement for growth media. Fetal bovine serum contains nutritional and embryonic growth promoting factors that are essential for a successful cell culture. Further, fetal bovine serum contains various small molecules, such as, e.g., amino acids, lipids, sugars, and hormones.

Fetal bovine serum (FBS) is the blood component obtained after coagulation and removal of cellular components. Its unique and comprehensive mix makes FBS one of the most important supplements for supporting cell growth and proliferation for in vitro cell culture.

Our fetal bovine serum for cell culture supports most cell lines and undergoes strict quality controls.

【产品介绍】

常规细胞培养需要添加血清来维持细胞的增殖能力，尽管血清可以满足一般细胞培养需求，但在进行营养成分优化和标记特定成分进行研究的实验中，仍需要去除某些特定的背景成分，常用的去除方法有碳吸附。

碳吸附胎牛血清用于体外培养，去除背景值以验证激素的作用效果，研究包括类固醇受体连接，细胞受体类固醇调节，各种组织激素分泌和甲状腺激素功能。生产过程中，使用炭和右旋糖苷去除血清中的激素。

血清通常用作细胞生长的添加物，拥有多种功能和用途，如：富含大分子蛋白，低分子量的营养素，作为氨基酸、碳水化合物、维生素的额外来源；提高介质的缓冲能力；可结合或中和有毒成分；减少细胞氧化应激，减少细胞凋亡；提供促进生长的因子，有助细胞适应培养环境。

【适用范围】

碳吸附胎牛血清常用于体外培养研究验证激素的作用效果。

适用于：培养干细胞、原代细胞、神经细胞、人类癌细胞、人类骨髓细胞、白血病细胞、人类胚肺成纤维细胞、人类脑神经细胞和瘤细胞等细胞。

【使用方法】

血清从冰冻区中取出后（切勿直接将血清从-20℃进入37℃解冻，这样因温度改变太大，容易造成蛋白质凝集而出现沉淀），置于2℃～8℃中使之融化，解冻过程中间歇地轻轻摇晃均匀，待全部溶解后再分装或直接使用。完全融解的血清从4℃拿出，按要求比例（5%-20%）加基础培养基配置成完全培养基，建议其置于4℃冰箱存放并于1-2周内用完。