Ⅱ型胶原α1(COL2α1)重组蛋白

YB194Rb011
Recombinant Nucleoporin 35kDa (NUP35)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
 Ⅱ型胶原α1(COL2α1)重组蛋白[ PROPERTIES ]
Residues: Thr132~Lys310 (Accession # Q8NFH5),
with two N-terminal Tags, His-tag and T7-tag. Host: E. coli
Subcellular Location: Nucleus, nuclear pore
complex. Nucleus membrane; Peripheral
membrane protein.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 9.5
 Ⅱ型胶原α1(COL2α1)重组蛋白Predicted Molecular Mass: 23.4kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
 Ⅱ型胶原α1(COL2α1)重组蛋白[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its
sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TGQSMFSPA SIGQPRKTTL
SPAQLDPFYT QGDSLTSEDH LDDSWVTVFG FPQASASYIL LQFAQYGNIL KHVMSNTGNW
MHIRYQSKLQ ARKALSKDGR IFGESIMIGV KPCIDKSVME SSDRCALSSP SLAFTPPIKT
LGTPTQPGST PRISTMRPLA TAYKASTSDY QVISDRQTPK